Statut | Confirmé |
Série | SEM-BESSON |
Domaines | cond-mat |
Date | Lundi 21 Mai 2007 |
Heure | 10:30 |
Institut | IMPMC |
Salle | Salle de conférence, Bâtiment 15, 140 rue de Lourmel, 75015 Paris |
Nom de l'orateur | Girard |
Prenom de l'orateur | Eric |
Addresse email de l'orateur | |
Institution de l'orateur | Synchrotron SOLEIL, BP48, 91192 GIF-sur-YVETTE Cedex |
Titre | What can be done with high pressures in bio-crystallography? |
Résumé | Until recently, only two crystal structures of small proteins at high pressure below 200 MPa generated in a Be cell were published [1,2]. The lack of structural data at high pressure on macromolecules was due mainly to the cumulated complexities of high-pressure containment and crystallography. A technical breakthrough was achieved with a set-up at the ESRF ID30/ID27 beamline combining a diamond anvil cell, ultra-short wavelength (0.33 Å) X-rays from undulators and a large imaging plate [3]. The accessible pressure range was increased by nearly one order of magnitude. The quality of diffraction data collected under high pressure achieved usual standards. After a brief introduction about the interest of high pressure in biology and about the specificity of macromolecular crystals, I will present some of the technical advances as well as some of the scientific results that we obtained [4,5]. Among these results, I will focus on the behaviour under pressure of a virus particle, a tetrameric protein as well as a small part of DNA, thus demonstrating that high pressure macromolecular crystallography can now be considered as a mature and general technique. [1] Kundrot C.E. & Richards F.M., 1987, J. Mol. Biol., 193, 157. [2] Urayama P., Phillips G.N. & Gruner S.M., 2002, Structure, 10, 51 [3] Fourme R. et al., 2003, Acta Cryst., D59, 1767. [4] Girard E., et al 2005, Biophys. J., 88, 3562. [5] Colloc'h N., et al, 2006, Biochim. Biophys. Acta, 1764, 391. |
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