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Statut |
Confirmé |
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Série |
LPS/ENS |
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Domaines |
physics |
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Date |
Mercredi 2 Mars 2011 |
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Heure |
11:00 |
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Institut |
LPS/ENS |
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Salle |
Conf. IV |
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Nom de l'orateur |
Berthoumieux |
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Prenom de l'orateur |
Hélene |
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Addresse email de l'orateur |
bhelene [at] pks [dot] mpg [dot] de |
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Institution de l'orateur |
Max-Planck-Institute for the Physics of Complex Systems |
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Titre |
Physical description of cellular shape : the role of actomyosin cortex tension in cell adhesion |
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Résumé |
Understanding the factors that direct tissue organization during development is one of the most
fundamental goals in developmental biology. Various hypotheses explain cell sorting and tissue
organization on the basis of the regulation of cell surface tension.
We distinguish two main factors governing the cohesion of an in vitro aggregate of cells, the formation
of dimers of adhesion proteins between the cells and the modification of the actomyosin cortex tension
at the adhesion zone. We try to evaluate the relative contribution of each of these phenomena. We
design a micropipette assay which permits the manipulation of individual cells held in suspension in
order to form controlled contact. We show that the size of the adhesion zone strongly depends on the
tension of the cell and prove that the cortical tension at the interface is modified. From a theoretical
point of view, we describe the equilibrium shape of the cell aggregate by modeling the cortex as a fluid
membrane or as a thin elastic shell depending on the characteristic life times of the observed shapes.
Together, experimental observations and model will allow us to quantify the adhesive and mechanical
properties of individual ectoderm, mesoderm and endoderm progenitor cells from gastrulating
zebrafish. |
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Numéro de preprint arXiv |
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